NEET Zoology Biotechnology Principles And Processes Class 12 Questions

Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :

Arrange the following steps of somatic hybridisation in a correct sequence: A. Digestion of cell walls. B. Isolation of naked protoplasts. C. Fusion of protoplasts to get hybrid protoplast. D. Isolation of single cells from two different varieties of plants. E. Growing of hybrid protoplast to form a new plant.

Which of the following statements are not true regarding restriction endonucleases? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the growth of bacteriophages in E. coli. C. They cut the DNA only at the centre of the palindromic sites. D. They remove nucleotides only from the ends of DNA fragments. E. They recognise specific palindromic base-pair sequences. Choose the answer from the options given below :

Insertion of a foreign DNA at BamHI site in an E.coli cloning vector pBR₃₂₂ results in the loss of antibiotic resistance towards:

Given below are two statements : Statement I : The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel. In the light of the above statements, choose the most appropriate answer from the options given below:

Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes B. DNA ligase C. DNA mutase D. DNA recombinase E. DNA polymerase Choose the correct answer from the options given below :

The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate. Given below are two statements about this method: Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies. Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies. In the light of the above statements, choose the most appropriate answer from the options given below :

Which of the following statements is incorrect?

The following diagram showing restriction sites in E.coli cloning vector pBR₃₂₂. Find the role of ‘X’ and ‘Y’ genes:

Which of the following is not a cloning vector?

Given below are two statements: Statement I: Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence. Statement II: Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site. In the light of the above statements, choose the most appropriate answer from the options given below:

Which of the following is not a desirable feature of a cloning vector?

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as:

Match List - I with List - II.

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

Plasmid pBR₃₂₂ has PstI restriction enzyme site within gene ampᴿ that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain

Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because :

DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as :

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

Choose the correct pair from the following: (1) Nucleases - Separate the two strands of DNA (2) Exonucleases - Make cuts at specific positions within DNA (3) Ligases - Join the two DNA molecules (4) Polymerases - Break the DNA into fragments

In gel electrophoresis, separated DNA fragments can be visualized with the help of:

DNA precipitation out of a mixture of biomolecules can be achieved by treatment with:

A gene whose expression helps to identify transformed cell is known as

The process of separation and purification of expressed protein before marketing is called

The DNA fragments separated on an agarose gel can be visualised after staining with

Which of the following is not required for any of the techniques of DNA fingerprinting available at present?

The Taq polymerase enzyme is obtained from

Which of the following is a restriction endonuclease?

A technique of micropropagation is :-

Which vector can clone only a small fragment of DNA?

Commonly used vectors for human genome sequencing are

Genes of interest can be selected from a genomic library by using:

During the process of isolation of DNA, chilled ethanol is added to: