NEET Zoology Biotechnology Principles And Processes Class 12 Questions

Polymerase chain reaction (PCR) amplifies DNA following the equation.

In the above represented plasmid an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

Identify the part of a bio-reactor which is used as a foam breaker from the given figure.

Given below are two statements : Statement I : The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel. In the light of the above statements, choose the most appropriate answer from the options given below:

Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes B. DNA ligase C. DNA mutase D. DNA recombinase E. DNA polymerase Choose the correct answer from the options given below :

The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate. Given below are two statements about this method: Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies. Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies. In the light of the above statements, choose the most appropriate answer from the options given below :

What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?

The capacity to generate a whole plant from any cell of the plant is called:

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

Which of the following are fused in somatic hybridization involving two varieties of plants?

Which of the following statements is incorrect?

The following diagram showing restriction sites in E.coli cloning vector pBR₃₂₂. Find the role of ‘X’ and ‘Y’ genes:

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

In gene gun method used to introduce alien DNA into host cells, microparticles of ________ metal are used.

Upon exposure to UV radiation, DNA stained with ethidium bromide will show

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence. A. Insertion of recombinant DNA into the host cell. B. Cutting of DNA at specific location by restriction enzyme. C. Isolation of desired DNA fragment. D. Amplification of gene of interest using PCR. Choose the correct answer from the options given below :

Which of the following is not a cloning vector?

Which one of the following statement is not true regarding gel electrophoresis technique?

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Polymerase chain reaction is used in DNA amplification Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation In the light of the above statements, choose the correct answer from the options given below:

Transposons can be used during which one of the following?

In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?

Given below are two statements: Statement I: Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence. Statement II: Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site. In the light of the above statements, choose the most appropriate answer from the options given below:

Which of the following is not a desirable feature of a cloning vector?

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as:

Match List - I with List - II.

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

Plasmid pBR₃₂₂ has PstI restriction enzyme site within gene ampᴿ that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain

Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because :

DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as :

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

Which of the following statements is not correct?

Choose the correct pair from the following: (1) Nucleases - Separate the two strands of DNA (2) Exonucleases - Make cuts at specific positions within DNA (3) Ligases - Join the two DNA molecules (4) Polymerases - Break the DNA into fragments

In gel electrophoresis, separated DNA fragments can be visualized with the help of:

Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?

Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the INCORRECT statement.

DNA precipitation out of a mixture of biomolecules can be achieved by treatment with:

The correct order of steps in Polymerase Chain Reaction (PCR) is

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

A gene whose expression helps to identify transformed cell is known as

The process of separation and purification of expressed protein before marketing is called

The DNA fragments separated on an agarose gel can be visualised after staining with

Which of the following is NOT a feature of the plasmids?

Which of the following is not required for any of the techniques of DNA fingerprinting available at present?

The Taq polymerase enzyme is obtained from

Which of the following is a restriction endonuclease?

A technique of micropropagation is :-

An analysis of chromosomal DNA using the Southern hybridization technique does not use