NEET Zoology Recombinant DNA MCQ Question
Which combinations of statements accurately describe aspects of recombinant DNA technology?
- Restriction enzymes can cut DNA at specific recognition sequences. 2. Plasmids can be used as vectors to introduce foreign DNA into host organisms. 3. DNA ligase is responsible for joining DNA fragments together.
- Exonucleases cut DNA at specific sequences. 2. Plasmids require no origin of replication to function. 3. Antibiotic resistance genes cannot be linked to plasmid vectors.
- Endonucleases are not involved in DNA manipulation. 2. Recombinant DNA cannot replicate on its own without a host. 3. Plasmids are only used for transferring RNA.
- Restriction enzymes are used to amplify RNA. 2. Selectable markers are not necessary in cloning vectors. 3. Recombinant DNA is created without the need for ligation.
Correct Answer
Detailed Explanation
Statement A is correct as it accurately describes the functions of restriction enzymes, plasmids as vectors, and the role of DNA ligase in recombinant DNA technology. Statement B is incorrect because exonucleases do not cut at specific sequences like endonucleases. Statement C is incorrect as endonucleases are integral to DNA manipulation and plasmids can replicate with the right conditions. Statement D is also incorrect because restriction enzymes are not used for RNA amplification and selectable markers are essential in cloning.
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