NEET Zoology Genetic Engineering MCQ Question
If a DNA fragment is cut using a restriction endonuclease and results in fragments of sizes 500 bp, 1500 bp, and 2000 bp, how would these fragments separate on an agarose gel during electrophoresis?
500 bp fragment moves furthest towards the anode.
2000 bp fragment moves furthest towards the anode.
All fragments move equally towards the anode.
1500 bp fragment moves furthest towards the anode.
Correct Answer
Detailed Explanation
DNA fragments separate based on size during gel electrophoresis, with smaller fragments moving faster and thus further. Hence, the 500 bp fragment moves furthest.
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