NEET Zoology Recombinant DNA MCQ Question
If a recombinant DNA is formed by inserting a gene of interest into a plasmid vector using a specific restriction enzyme, and the plasmid has a total length of 6000 base pairs after insertion, how many base pairs were originally in the vector if the gene of interest is 1500 base pairs long?
4500 base pairs
7500 base pairs
6000 base pairs
1500 base pairs
Correct Answer
Detailed Explanation
The total length of the recombinant DNA is the sum of the original vector length and the gene of interest. Since the recombinant DNA is 6000 base pairs and the gene of interest is 1500 base pairs, the original vector length must be 6000 - 1500 = 4500 base pairs.
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