RE-NEET Zoology Biotechnology – Principles and Processes Class 12 Questions

Match List-I with List-II.

During PCR, primers bind to the DNA strands in the ______ step.

Given below are two statements :

Statement I: Plasmids are autonomously replicating DNA.

Statement II: Plasmids are extrachromosomal DNA.

In the light of the above statements, choose the most appropriate answer from the options given below :

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R. Assertion A: In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is for plant cells. Reason R: Isolation of genetic material needs disruption of cells. In the light of the above statements, choose the most appropriate answer from the options given below:

Identify the incorrect statement related to gel electrophoresis.

Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR₃₂₂ cloning vector.

Which of the following are correct about EcoR₁?

A. Cut the DNA with blunt end

B. Cut the DNA with sticky end

C. Recognise a specific palindromic sequence

D. Cut the DNA between the base G and A when encounters the DNA sequence ‘GAATTC’

E. Exonuclease Choose the correct answer from the options given below:

Following are the steps involved in the process of PCR.

A. Annealing

B. Amplification (~1 billion times)

C. Denaturation

D. Treatment with Taq polymerase and deoxynucleotides

E. Extension Choose the correct sequence of steps of PCR from the options given below: