RE-NEET2024Zoology-Biotechnology

RE-NEET 2024 Zoology Polymerase Chain Reaction MCQ Question

Type: MCQ-conceptual-Medium-Class 12

Following are the steps involved in the process of PCR.

A. Annealing

B. Amplification (~1 billion times)

C. Denaturation

D. Treatment with Taq polymerase and deoxynucleotides

E. Extension Choose the correct sequence of steps of PCR from the options given below:

A

C → A → D → E → B

B

A → B → E → D → C

C

A → C → E → D → B

D

D → B → E → C → A

Correct Answer

Option A

Detailed Explanation

The correct sequence for PCR is Denaturation, Annealing, Extension, followed by Amplification. This sequence ensures that the DNA is first denatured, primers anneal, and then the polymerase extends the DNA strands.

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