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RE-NEET2024Zoology-Cloning Vectors

RE-NEET 2024 Zoology Cloning Vectors MCQ Question

Type: MCQ-fact-Easy-Class 12

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR₃₂₂ cloning vector.

A

Bam HI and Sal I

B

Sal I and Pst I

C

Pst I and Pvu I

D

Pvu I and Bam HI

Correct Answer

Option A

Detailed Explanation

The pBR₃₂₂ plasmid contains restriction sites for Bam HI and Sal I within the tetracycline resistance gene, which can be used for cloning purposes.

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