NEET Zoology Recombinant DNA MCQ Question
If a recombinant plasmid is constructed using a vector with an origin of replication that supports a high copy number, how many copies of the plasmid can be expected in a bacterial cell if the copy number of the vector is 100?
Approximately 100 copies
Approximately 10 copies
Approximately 1000 copies
Approximately 1 copy
Correct Answer
Detailed Explanation
The vector's origin of replication determines the copy number of the recombinant plasmid. A high copy number vector with a copy number of 100 will produce approximately 100 copies within the host cell.
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