NEET Zoology Recombinant DNA MCQ Question
If a recombinant DNA is inserted into a vector with an origin of replication that supports a high copy number, and each bacterial cell can contain up to 100 copies of this plasmid, how many copies of the recombinant DNA can be expected in a culture containing 1 million bacterial cells?
100 million
10 million
1 million
500 million
Correct Answer
Detailed Explanation
The origin of replication in a vector determines the copy number of the plasmid. With a high copy number plasmid supporting up to 100 copies per cell, a culture of 1 million cells can have 1 million x 100 = 100 million copies of the recombinant DNA.
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