NEET 2021 Zoology Recombinant DNA Technology MCQ Question
Plasmid pBR₃₂₂ has PstI restriction enzyme site within gene ampᴿ that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain
it will not be able to confer ampicillin resistance to the host cell.
the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.
it will lead to lysis of host cell.
it will be able to produce a novel protein with dual ability.
Correct Answer
Detailed Explanation
Inserting a gene at the PstI site within the ampᴿ gene disrupts the gene, making the plasmid unable to confer ampicillin resistance.
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