NEET2021Zoology-Genetics
NEET 2021 Zoology PCR MCQ Question
Type: MCQ-conceptual-Medium-Class 12
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
A
Annealing
B
Extension
C
Denaturation
D
Ligation
Correct Answer
Option C
Detailed Explanation
Denaturation requires high temperature to separate DNA strands. Without it, the process cannot start.
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