NEET Zoology Genetic Engineering MCQ Question
In the process of introducing recombinant DNA into bacteria, cells are first incubated on ice and then exposed to a brief heat shock at 42°C. What is the importance of the heat shock step in terms of bacterial cell transformation efficiency?
It increases the permeability of the bacterial cell wall.
It enhances the replication rate of the introduced DNA.
It helps in the inactivation of antibiotic resistance genes.
It stabilizes the recombinant DNA structure.
Correct Answer
Detailed Explanation
The heat shock step increases the permeability of the bacterial cell wall, allowing the recombinant DNA to enter the bacterial cell, as mentioned in the NCERT context.
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