NEET Zoology Genetic Engineering MCQ Question
If a plasmid with an origin of replication supporting a high copy number is used for cloning in a host cell, and each cell has 100 copies of the plasmid, how many copies of the target DNA can be recovered from 1000 transformed cells?
100,000 copies
1,000,000 copies
10,000 copies
1,000 copies
Correct Answer
Detailed Explanation
Each transformed cell contains 100 copies of the plasmid. With 1000 cells, the total copies of the plasmid, and hence the target DNA, would be 100,000 (100 copies/cell × 1000 cells).
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