NEET Zoology (general) MCQ Question
Which of the following combinations correctly describe the features and processes involved in the cloning of DNA into a vector?
Origin of replication ensures high copy number; Selectable marker identifies transformants; Restriction enzymes cut DNA at specific locations.
Origin of replication prevents replication of linked DNA; Selectable marker is unnecessary; Ligase cuts DNA at specific sites.
Selectable marker is the same as origin of replication; Restriction enzymes are used only in downstream processing; Ligase is used to cut DNA.
Origin of replication is irrelevant; Selectable marker helps in transformation; Restriction enzymes are only for DNA amplification.
Correct Answer
Detailed Explanation
The correct combination in option A accurately reflects the requirements for cloning into a vector, as stated in the NCERT context. The origin of replication is crucial for controlling the copy number, and selectable markers facilitate the identification of successful transformants, while restriction enzymes are essential for cutting DNA at specific sites.
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