NEET 2026 Zoology DNA Fingerprinting MCQ Question
Arrange the following steps of DNA fingerprinting in a correct sequence.
Isolation of DNA and its digestion by restriction endonucleases.
Hybridisation using a labelled VNTR probe.
Transferring of separated DNA fragments to synthetic membranes.
Detection of hybridised DNA fragments by autoradiography.
Correct Answer
Detailed Explanation
The following is the correct sequence of steps of DNA fingerprinting:
A. Isolation of DNA and its digestion by restriction endonuclease.
C. The separated DNA fragments are transferred to synthetic membranes made of nylon or nitrocellulose.
E. DNA fragments are separated based on their size by the technique of gel electrophoresis.
B. Probes made complementary to the VNTR locus are allowed to hybridise with the DNA fragments.
D. Finally, the hybridised DNA fragments are detected under X⁻rays in a technique called autoradiography.
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