NEET2024Zoology-Cloning Vectors
NEET 2024 Zoology pBR322 Restriction Sites MCQ Question
Type: MCQ-conceptual-Hard-Class 12
The following diagram showing restriction sites in E.coli cloning vector pBR₃₂₂. Find the role of ‘X’ and ‘Y’ genes:

A
The gene ‘X’ is for protein involved in replication of Plasmid and ‘Y’ for resistance to antibiotics.
B
Gene ‘X’ is responsible for recognition sites and ‘Y’ is responsible for antibiotic resistance.
C
The gene ‘X’ is responsible for resistance to antibiotics and ‘Y’ for protein involved in the replication of Plasmid.
D
The gene ‘X’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of Plasmid.
Correct Answer
Option D
Detailed Explanation
In pBR₃₂₂, the gene ‘X’ is typically associated with antibiotic resistance, while ‘Y’ is involved in plasmid replication.
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