NEET2024Zoology-Cloning Vectors

NEET 2024 Zoology pBR322 Restriction Sites MCQ Question

Type: MCQ-conceptual-Hard-Class 12

The following diagram showing restriction sites in E.coli cloning vector pBR₃₂₂. Find the role of ‘X’ and ‘Y’ genes:

Question diagram
A

The gene ‘X’ is for protein involved in replication of Plasmid and ‘Y’ for resistance to antibiotics.

B

Gene ‘X’ is responsible for recognition sites and ‘Y’ is responsible for antibiotic resistance.

C

The gene ‘X’ is responsible for resistance to antibiotics and ‘Y’ for protein involved in the replication of Plasmid.

D

The gene ‘X’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of Plasmid.

Correct Answer

Option D

Detailed Explanation

In pBR₃₂₂, the gene ‘X’ is typically associated with antibiotic resistance, while ‘Y’ is involved in plasmid replication.

Found an issue with this question?