NEET 2024 Zoology pBR322 Restriction Sites MCQ Question
The following diagram showing restriction sites in E.coli cloning vector pBR₃₂₂. Find the role of ‘X’ and ‘Y’ genes:

The gene ‘X’ is for protein involved in replication of Plasmid and ‘Y’ for resistance to antibiotics.
Gene ‘X’ is responsible for recognition sites and ‘Y’ is responsible for antibiotic resistance.
The gene ‘X’ is responsible for resistance to antibiotics and ‘Y’ for protein involved in the replication of Plasmid.
The gene ‘X’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of Plasmid.
Correct Answer
Detailed Explanation
In pBR₃₂₂, the gene ‘X’ is typically associated with antibiotic resistance, while ‘Y’ is involved in plasmid replication.
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