NEET2021Zoology-Biotechnology: Principles and Processes
NEET 2021 Zoology Recombinant DNA Technology MCQ Question
Type: MCQ-conceptual-Hard-Class 12
Plasmid pBR₃₂₂ has PstI restriction enzyme site within gene ampᴿ that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain
A
it will not be able to confer ampicillin resistance to the host cell.
B
the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.
C
it will lead to lysis of host cell.
D
it will be able to produce a novel protein with dual ability.
Correct Answer
Option A
Detailed Explanation
Inserting a gene at the PstI site within the ampᴿ gene disrupts the gene, making the plasmid unable to confer ampicillin resistance.
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