NEET 2025 Zoology Plasmid Vectors MCQ Question
In the above represented plasmid an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
Using ampicillin & tetracyclin containing medium plate.
Blue color colonies will be selected.
White color colonies will be selected.
Blue color colonies grown on ampicillin plates can be selected.
Correct Answer
Detailed Explanation
Insertion of DNA at the EcoRI site disrupts the lacZ gene, leading to white colonies in the presence of X⁻gal, indicating successful recombination.
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