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NEET2022Botany-Messelson & Stahl Experiment

NEET 2022 Botany Messelson & Stahl Experiment MCQ Question

Type: MCQ-numerical-Hard-Class 12

Ten E.coli cells with ¹⁵N⁻dsDNA are incubated in medium containing ¹⁴N nucleotide. After 60 minutes how many E.coli cells with have DNA totally free from ¹⁵N?

A

60 cells

B

80 cells

C

20 cells

D

40 cells

Correct Answer

Option A

Detailed Explanation

Chapter: Molecular Basis of Inheritance

Class: 12 Topic: Meselson & Stahl Experiment Difficulty: 🔴 Hard

✅ Ans: A — 60 cells

E. coli generation time = 20 min.

After 60 min:

60/20=3 generations60/20=3\text{ generations}

Starting with 10 cells:

10×23=80 cells10\times2^3=80\text{ cells}

After 3 generations of semiconservative replication, 34\frac34 of the DNA molecules are completely 14N^{14}N.

80×34=6080\times\frac34=60

🧠 NEET Trick

After nn generations: total cells = N02nN_0 2^n. After the first generation, only hybrid DNA exists; subsequently the fraction of completely light DNA increases.

Answer: 60 cells

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