STANDARDZoology-Genetic Engineering

STANDARD Zoology Bacterial Transformation MCQ Question

Type: MCQ-conceptual-Medium

Significance of 'heat shock' method in bacterial transformation is to facilitate

A

Binding of DNA to the cell wall

B

Uptake of DNA through membrane transport proteins

C

Uptake of DNA through transient pores in the bacterial cell wall

D

Expression of antibiotic resistance gene

Correct Answer

Option C

Detailed Explanation

Heat shock transformation uses a calcium rich environment provided by calcium carbonate to counteract the electrostatic repulsion between the plasmid DNA and bacterial cellular membrane.

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