STANDARD Zoology Bacterial Transformation MCQ Question
Significance of 'heat shock' method in bacterial transformation is to facilitate
Binding of DNA to the cell wall
Uptake of DNA through membrane transport proteins
Uptake of DNA through transient pores in the bacterial cell wall
Expression of antibiotic resistance gene
Correct Answer
Detailed Explanation
Heat shock transformation uses a calcium rich environment provided by calcium carbonate to counteract the electrostatic repulsion between the plasmid DNA and bacterial cellular membrane.
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