STANDARD Botany Enzymes in rDNA MCQ Question

Formation of recombinant DNA (rDNA) primarily involves the use of a vector, which is a DNA molecule that can carry foreign genetic material into a host cell. Vectors, such as plasmids or viruses, facilitate the insertion of the desired gene into a host organism for cloning or expression.

Option A, DNA ligase, is involved in sealing nicks in the DNA backbone but does not create rDNA on its own. Option B, molecular scissors (restriction enzymes), are used to cut DNA at specific sequences but do not encompass the entire process of rDNA formation. Option D, DNA polymerase, is responsible for synthesizing new DNA strands but is not directly involved in the initial formation of rDNA.