STANDARD Botany PCR MCQ Question
Which process is represented in following diagram?
PCR
DNA finger printing
CTAB
DNA spooling
Correct Answer
Detailed Explanation
DNA spooling is the process of isolating DNA from a solution by using a spool or stick to physically collect the visible strands of DNA that precipitate out when alcohol, such as ethanol or isopropanol, is added to a DNA solution. This method relies on the solubility properties of DNA in aqueous solutions (H₂O) versus alcohol, allowing for the extraction of pure DNA.
In contrast, PCR (Polymerase Chain Reaction) (Option A) is a technique used to amplify specific DNA sequences, while DNA fingerprinting (Option B) involves analyzing unique patterns in an individual's DNA for identification purposes. CTAB (Option C) refers to a method for DNA extraction using cetyltrimethylammonium bromide, which is different from the physical collection of DNA strands seen in spooling.
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