STANDARDBotany-Genetic Engineering

STANDARD Botany PCR MCQ Question

Type: MCQ-numerical-Medium

If we use a single molecule of dsDNA and pass it through 3 PCR cycles. ______ many ds DNA copies will be obtained in the end

A

8

B

16

C

24

D

48

Correct Answer

Option A

Detailed Explanation

In PCR (Polymerase Chain Reaction), each cycle ideally doubles the amount of dsDNA. Starting with one molecule of dsDNA, after 3 cycles, the formula for the number of copies is 2^n, where n is the number of cycles. Thus, 2^3 = 8 copies are produced. Options B (16), C (24), and D (48) are incorrect because they miscalculate the exponential growth of DNA copies; they do not follow the doubling principle inherent in PCR.

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