AIPMT MAINS2012Zoology-Biotechnology
AIPMT MAINS 2012 Zoology Polymerase Chain Reaction MCQ Question
Type: MCQ-conceptual-Medium-Class 12
The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct 'identification together with what it represents'?
A
B - Denaturation at a temperature of about 98°C separating the two DNA strands.
B
A - Denaturation at a temperature of about 50°C
C
C - Extension in the presence of heat stable DNA polymerase
D
A - Annealing with two sets of primers
Correct Answer
Option C
Detailed Explanation
Expansion proceeds at 72°C in the presence of Taq DNA polymerase in PCR, which corresponds to the extension step.
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