AIIMS 2019 Zoology Plasmids MCQ Question
Assertion: E.coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.
Reason: Recognition site for BamHI is present in region of pBR322.
If both assertion and reason are true and reason is the correct explanation of assertion.
If both assertion and reason are true but reason is not the correct explanation of assertion.
If assertion is true but reason is false.
If both assertion and reason are false.
Correct Answer
Detailed Explanation
When an insert is introduced at the BamHI recognition site within the terᵣ region of plasmid pBR₃₂₂, it disrupts the coding sequence for tetracycline resistance, rendering the gene non-functional. Consequently, bacteria harboring this recombinant plasmid lose their ability to resist tetracycline, making them sensitive to the antibiotic. The other options are not applicable as they do not provide relevant information regarding the impact of the insert on tetracycline resistance. This illustrates the principle of gene disruption in recombinant DNA technology, where insertional inactivation can be used to identify successful transformations.
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