AIIMS2004Botany-Molecular Techniques
AIIMS 2004 Botany Southern Blotting MCQ Question
Type: MCQ-conceptual-Medium-Class 12
What is the first step in the Southern Blot technique?
A
denaturation of DNA on the gel for hybridization with specific probe
B
production of a group of genetically identical cells
C
digestion of DNA by restriction enzyme
D
isolation of DNA from a nucleated cell such as the one from the scene of crime
Correct Answer
Option D
Detailed Explanation
Southern blotting was named after Edward M. Southern who developed this procedure in Edinburgh University in the 1970’s.
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