AIIMS 2019 Biology Requirements for Tissue Culture MCQ Question
What are the requirements in tissue culture
Hormones like auxin, cytokinin, agar-agar
Inorganic salt, vitamin, amino acid only
Carbon source like sucrose only
All of these
Correct Answer
Detailed Explanation
Tissue culture requires a combination of essential components to support the growth and development of plant cells. This includes hormones such as auxins and cytokinins to regulate growth, agar-agar as a gelling agent, inorganic salts for nutrient supply, vitamins for metabolic functions, amino acids for protein synthesis, and a carbon source like sucrose to provide energy. While options B and C mention important elements, they do not encompass the full range of requirements necessary for successful tissue culture, making option D the most comprehensive choice.
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